Generator

Part:BBa_K1361006:Design

Designed by: Shoujie Sun   Group: iGEM14_Tianjin   (2014-10-07)


Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 707
    Illegal NheI site found at 730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.


Source

CsgAC and CsgBtrunc were collected by PCR from genome of E coli DH5alpha respectively.

References