Composite

Part:BBa_K1361000:Design

Designed by: Shoujie Sun   Group: iGEM14_Tianjin   (2014-10-03)


Curli Fiber generator under Pbad induction with positive regulation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1545
    Illegal NheI site found at 1568
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG. And for nano gold absorbability, 6xLinker+2xCys(GGCAGCGGTGGCAGCGGTGGCGGT TGTTGC) and LPFFD(CTGCCGTTCTTTGAT) were added at the C-treminal of CsgA protein respectively. CsgA with7xhis tag at C-treminal were also used for Au-NTA-Ni absorbing.


Source

CsgAC,CsgB collected by PCR from genome of E coli DH5alpha respectively. Other genes are collected from Biobricks Attribution Kit.

References