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Composite

Part:BBa_K136031:Design

Designed by: alexandra bouaziz   Group: iGEM08_Paris   (2008-10-25)


RBS-lasI - ECFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1604
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1166
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

psB1A2 are digested by SpeI PstI (BV),then the promotor(insert) was cloned into the J61002 plasmid containing gFP tripart .


Source

Assembly of different parts from the registry.

References