Deleted
Sample Discontinued
Experience: None
Not Used
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Coding

Part:BBa_K136005:Design

Designed by: Cyprien Maisonnier   Group: iGEM08_Paris   (2008-08-28)


FlhDC gene from


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 860
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 860
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 860
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 860
    Illegal AgeI site found at 648
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primers used to amplify this sequence are :

  • Forward :

GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTA

  • Reverse :

GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAACAGCCTGTACTCTCTGTTCATCC



Source

This part is from E. coli" K 12 strain MG1655 genome.

References