Composite

Part:BBa_K1357009:Design

Designed by: Matthew Tucker   Group: iGEM14_Virginia   (2014-10-09)

amilCP Reporter (RBS+Coding+Term)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added a ribosomal binding site (B0034) and a terminator (B0015) to the tsPurple coding sequence (K592009)[1] that was previously cloned by Uppsala 2013

Source

Coding sequence; BBa_K592009[2]. BBa_K592009 is derived from Acropora millepora. It's DNA was originally provided by Jeffery Miller from UCLA.

References

Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.

https://parts.igem.org/Part:BBa_K592009:Design