Part:BBa_K1357004:Design

Constitutive NhaR Expression Device

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 236
  

Design Notes

This constitutive promoter was chosen for its high activity. Similarly the RBS and Terminator were chosen because of their reliability and efficiency.

Source

The coding sequence for NhaR was taken from the E. coli K-12 genome and the other parts were received in the parts distribution kit.