Part:BBa_K1356000:Experience

Experimental Data (13 October 2014)

The table below shows the initial set-up of tests run. We decided to not run the test where we would let the biofilm sit for 72 hours before introducing the enzyme but instead used those tubes as a check to see whether enough time had passed to allow the biofilm to adhere to sides of the tube. We would check biofilm adherence by inverting the tube and tapping it on the table twice. Once we were sure the biofilm had had enough to set, we tested the rest of the tubes from the actual test (what we had meant to let run for 48 hours but instead allows to run for 7 days). From the tests run in triplicate, we were unable to reject our initial hypothesis - that of the purified amylase enzyme being able to degrade pre-existing biofilm. We saw that the test tubes that had the enzyme would drop clumps of biofilm when tapped on the table, whereas the tubes with no enzyme or solution and those with the glycerol/elution buffer solution did not release any biofilm when tapped on the table. We will be refining the test and duplicating the results.

Please refer to the following links to see tables and images related to this test:

http://2014.igem.org/Team:BYU_Provo/Modeling

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