![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Terminal D
Part:BBa_K1354002:Design
Designed by: Hua Joe Fung, Jiwoon Park, Alexa Orrico, Wilfrido Castillo Group: iGEM14_Cooper_Union (2014-10-03)
Bovine Terminal Deoxynucleotidyl Transferase: codon optimized for expression in E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1384
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 933
Design Notes
This sequence codes for bovine terminal deoxynucleotidyl transferase. It has been codon optimized for expression in E. coli. Common restriction sites have been mutated out of the sequence. An Nde1 restriction site was added to the 5' end so that it could also be cloned into the expression vector pET28b+.
Source
http://www.ncbi.nlm.nih.gov/protein/29135289
References
Wee Liang Kuan, Joma Joy, Ng Fui Mee, et al: Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli. Biochemistry Insights [2010]:[3] [41]-[46]