Part:BBa_K1352011:Design

Ag43+FLAG+MCS for surface display of foreign peptides

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 442
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 745
  Illegal NgoMIV site found at 1969
  Illegal NgoMIV site found at 2479
  Illegal NgoMIV site found at 2500
  Illegal AgeI site found at 1495
  Illegal AgeI site found at 2239
  Illegal AgeI site found at 2653
  Illegal AgeI site found at 2895
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

FLAG epitope tag flanked by BglII and HindIII multiple cloning sites was designed to be in frame of the Ag43 ORF; it is optimized for E.coli codon bias.

Source

This part was PCR amplified from BioBrick Bba_K1352000 and subjected to In-Fusion (Clonetech) to insert in-frame FLAG+MCS