Composite
Ag43+FLAG
Part:BBa_K1352001:Design
Designed by: Martyna Sroka, Ana-Maria Cujba Group: iGEM14_Aberdeen_Scotland (2014-10-02)
Ag43+FLAG+MCS under the control of pBAD promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1678
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1981
Illegal NgoMIV site found at 3205
Illegal NgoMIV site found at 3715
Illegal NgoMIV site found at 3736
Illegal AgeI site found at 979
Illegal AgeI site found at 2731
Illegal AgeI site found at 3475
Illegal AgeI site found at 3889
Illegal AgeI site found at 4131 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
FLAG tag epitope flanked by multiple cloning sites was designed to be in-frame with Ag43 ORF; it is optimized for E.coli codon bias.
Source
This BioBrick was PCR amplified from Bba_K1352000; in-frame FLAG tag epitope flanked by multiple cloning sites was inserted via In-Fusion (Clonetech).