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Part:BBa_K1351038:Design

Designed by: Nikolai Peschek   Group: iGEM14_LMU-Munich   (2014-10-06)

P3-promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Several mutated illegal restriction sites. The mutated codons have been optimized regarding the codon usage of Bacillus subtilis.


Source

This part was generated by amplification from artificially synthesized DNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

P3_fwd: GATCGAATTCGCGGCCGCTTCTAGAGGTACAATCTTTTATCATTATATTGCCTAAC

P3_rev: GATCACTAGTACTCTGTGATCTAGTTATATTAAAACATGC

Annealing sequences are written in bold.

References

Reynolds, J. and S. Wigneshweraraj (2011). "Molecular Insights into the Control of Transcription Initiation at the Staphylococcus aureus agr operon." Journal of Molecular Biology 412(5): 862-881.