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Coding

Part:BBa_K1351002:Design

Designed by: Mona Dotzler   Group: iGEM14_LMU-Munich   (2014-10-03)

Chondroitin 4 sulfotransferase-derived peptide binding to NADH Oxidase of S. pneumoniae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For generation of the part's nucleotide sequence, the amino acid sequence of the peptide (Muchnik et. al., 2013) was reverse transcribed with regard to the codon usage of Bacillus subtilis.

Oligos were designed as precut by EcoRI and PstI to shorten the synthesized fragments and to simplify the cloning into pSB1C3.

Source

The part was generated by annealing of artificially synthesized oligonucletides:

CSP_ENX_SD_Ngo_precutEP_fwd: AATTCgcggccgctTCTAGAgtaaggaggaaGCCGGCATG GCGAACCACACATCACTTCCGGCGTCAGCGAGAGAA ACCGGTtaatACTAGTagcggccgCTGCA

CSP_SNP_Age_precutEP_rev: GcggccgctACTAGTattaACCGGT TTCTCTCGCTGACGCCGGAAGTGATGTGTGGTTCGC CATGCCGGCttcctccttacTCTAGAagcggccgcG

References

Muchnik, L., A. Adawi, A. Ohayon, S. Dotan, I. Malka, S. Azriel, M. Shagan, M. Portnoi, D. Kafka, H. Nahmani, A. Porgador, J. M. Gershoni, D. A. Morrison, A. Mitchell, M. Tal, R. Ellis, R. Dagan and Y. M. Nebenzahl. "Nadh Oxidase Functions as an Adhesin in Streptococcus Pneumoniae and Elicits a Protective Immune Response in Mice." PLoS One 8, no. 4 (2013): e61128. [http://www.ncbi.nlm.nih.gov/pubmed/23577197 PubMed]