Regulatory

Part:BBa_K1346002:Design

Designed by: Eleanor Amidei   Group: iGEM14_UCSF_UCB   (2014-07-15)

pTEF1(m7) - constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169


Design Notes

m7 has a difference of about 25bp from pTEF1


Source

Mutant of pTEF1 which is endogenous to the S. Cerevisiae genome.

References

Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae Elke Nevoigt1,2, Jessica Kohnke2, Curt R. Fischer1, Hal Alper1, Ulf Stahl2 and Gregory Stephanopoulos1,*

http://aem.asm.org/content/72/8/5266.long