Signalling

Part:BBa_K1343015:Design

Designed by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon   Group: iGEM14_Technion-Israel   (2014-09-27)


PT7-LuxI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
    Illegal XhoI site found at 25
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20


Design Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other


Source

Placeholder

References