Regulatory
Part:BBa_K1343005:Design
Designed by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon Group: iGEM14_Technion-Israel (2014-09-27)
PCI-tetR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 58
Illegal XhoI site found at 51 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other
Source
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