![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1333202:Design
Designed by: Yanwen Xie Group: iGEM14_SYSU-China (2014-09-23)
lambda cI-LGF2(R74A)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 712
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The LGF2 is mutated from CGA to GCA at site 74.
Source
It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).