Coding

Part:BBa_K1333110:Design

Designed by: Zhang Fangyingnan   Group: iGEM14_SYSU-China   (2014-10-17)


recA730 CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

two synonymous mutations to remove the inherent EcorI and PstI site


Source

E.coli genome

References