Part:BBa_K1333104

RBS DNAQ926

The product of DNAQ926 can raise the mutation rate in E.coli.

Usage and Biology

NA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication. DNAQ926 is a mutant of DNAQ, which doesn’t have the proofreading function but still can bind to the polymerase III complex. If DNAQ926 is expressed in the E.coli, it can compete the internal product of DNAQ, which can lead to the mutation during DNA replication.

SOS error prone bypass

For more information please visit our wiki

http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutation

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 192
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 64
  Illegal AgeI site found at 553
  
• 1000
  COMPATIBLE WITH RFC[1000]