Intermediate

Part:BBa_K133123:Design

Designed by: Jan Lonzaric   Group: iGEM08_Slovenia   (2008-10-29)

CMV-SS-HA-UreB-gyrEC-linker-(TMTIR)TLR9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2918
    Illegal BamHI site found at 1790
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1012
    Illegal AgeI site found at 2190
    Illegal AgeI site found at 2590
    Illegal AgeI site found at 2728
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3572
    Illegal SapI.rc site found at 898


Design Notes

Cloned into pSB1AK3 vector.

Source

References