Part:BBa_K133049

TetR-CF-UreB-RGD-Histop

TetR repressible promoter with ribosomal binding site.

Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal) fused with UreB. Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production.

Urease subunit beta is an important pathogenicity factor for H. pylori since it enables the bacteria the breakdown of urea into ammonia and carbon dioxide, thus artificially buffering the acidic enviroment in the stomach.

Since the protein was designed to be produced, we additionally added RGD-Hisstop tag.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1073
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 295
  Illegal AgeI site found at 1473
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1780
  Illegal SapI.rc site found at 181