Coding

Part:BBa_K133046:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)

CF215


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 571
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 128
    Illegal BamHI site found at 639
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was cloned into pSB1.AK3 with terminator for use in bacterial cells (surface expression).

Source

We used polimerase chain reaction from chimeric flagellin [1].

References