Part:BBa_K133029:Design

HpaA-multiHP-RGD-Histop

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 139
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 170
  Illegal BglII site found at 266
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1326
  

Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

HpaA and Multiepitope were ordered from GeneArt. Rgd-Hisstop was composed with annealing.