Intermediate
Part:BBa_K133027:Design
Designed by: Ana Lasic Group: iGEM08_Slovenia (2008-10-29)
HpaA-UreB-RGD-Histop
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 139
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 170
Illegal BglII site found at 266
Illegal BamHI site found at 1799 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1021
Illegal AgeI site found at 2199 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2506
Illegal SapI.rc site found at 907
Design Notes
The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).
Source
HpaA and LPP were ordered from GeneArt, so were primers for RGD-Histop. RGD-Histop was constructed with annealing.