Composite

Part:BBa_K133024:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)

CMV-ss-CF-UreB-RGD-Histop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1948
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1505
    Illegal BamHI site found at 2016
    Illegal BamHI site found at 3305
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1042
    Illegal AgeI site found at 2527
    Illegal AgeI site found at 3705
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4012
    Illegal SapI.rc site found at 2413


Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

Plasmid DNA

References