Intermediate

Part:BBa_K133017:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)

mCherry-RGD-Histop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 721


Design Notes

none

The CF-multiHP-RGD-Histop was cloned into pSB1.AK3 without terminator for use in bacterial cells.

Source

plasmid: Red Flourescence Protein was obtaind from previousely designed biobrick of 2007 iGEM team Ljubljana containing Red Flourescence Protein with added nuclear localisation signal and mutated PstI restriction site.

References