Part:BBa_K133015:Design

CF215-mCherry-RGD-Histop

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 571
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 128
  Illegal BamHI site found at 639
  Illegal BamHI site found at 1603
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1638
  

Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

plasmid DNA cloned or modified in our laboratory.