Intermediate
Part:BBa_K133012:Design
Designed by: Ana Lasic Group: iGEM08_Slovenia (2008-10-29)
CF213-UreB-linker-CF215-RGD-Histop
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2958
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1655
Illegal BamHI site found at 2515
Illegal BamHI site found at 3026 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
Illegal AgeI site found at 877
Illegal AgeI site found at 2055 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3309
Illegal SapI.rc site found at 763
Design Notes
The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).
Source
plasmid DNA (our other parts)