Part:BBa_K133012
CF213-UreB-linker-CF215-RGD-Histop
This part is composed of
1. CF213, which is the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215) In combination with CF215 it forms a TLR5 activating protein.
2. UreB, which is beta subunit of H. pylori urease that we used as an antigen
3. linker, which is 8 AA long flexible peptide useful when combining various domains to conserve their 3D integrity
4. CF215, which is the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coli flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215). In combination with CF213 it forms a TLR5 activating protein.
5. RGD - His stop, which is useful for protein production.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2958
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1655
Illegal BamHI site found at 2515
Illegal BamHI site found at 3026 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
Illegal AgeI site found at 877
Illegal AgeI site found at 2055 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3309
Illegal SapI.rc site found at 763
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