Intermediate

Part:BBa_K133003:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)

CF-UB33


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 764
    Illegal BamHI site found at 1275
    Illegal BamHI site found at 1627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

The composite parts of chimeric protein were amplificated by PCR reaction from E. coli and H. pylori genomic DNA respecively. UB33 is a synthetic gene ordered from GeneArt.

References