Plasmid_Backbone

Part:BBa_K1323017:Design

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-04)

E. coli - Staphylococcus Shuttle Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 4855
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4855
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4861
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4855
    Illegal BglII site found at 2978
    Illegal BamHI site found at 178
    Illegal BamHI site found at 186
    Illegal XhoI site found at 3839
    Illegal XhoI site found at 4731
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4855
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4855
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4870
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 5657
    Illegal AgeI site found at 5769
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.