Generator

Part:BBa_K1322231:Design

Designed by: Jarrod Shilts   Group: iGEM14_Vanderbilt   (2014-10-16)


Santalene Synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Two sites on the original gene sequence had to be changed by selected sense mutation codons in order to make the part RFC10 compatible. The sequence was codon optimized to maximize expression in Saccharomyces cerevisiae.

Source

The sequence comes from genomic DNA taken from the santalum genus of plant. It was codon optimized for expression in Saccharomyces cerevisiae. The part also contains a yeast consensus sequence before the start codon, a C-terminal strep tag, and biobrick prefixes and suffixes.

References