Coding
Part:BBa_K1321361:Design
Designed by: Chris N Micklem Group: iGEM14_Imperial (2014-10-08)
CBDcenA with linker fused to NiBP driven by LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fusion protein was cloned together using the NgoMIV and AgeI sites.
The promotor was cloned onto the protein via the XbaI/SpeI sites.
Source
CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.
Our NiBP came from BBa_K1151001.
Our LacI promotor came from BBa_J04500.