Coding

Part:BBa_K1321351:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)


CBDcenA with linker fused to NiBP in Freiburg format (RFC 25)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

Source

Our NiBP came from BBa_K1151001. CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

References