Coding

Part:BBa_K1321338:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)

T7 Expression vector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR was performed using BBa_I746909 as a template with the following primers:

Forward Primer: 5'-TAC TAG TAG CGG CCG CTG CAG-3' Reverse Primer: 5'-TTT CTC CTC TTT CTC TAG TAT CTC CC-3'


Source

PCR template was BBa_I746909 which was miniprepped from 2014 distribution Plate 3 Well 8M