Composite

Part:BBa_K1321332:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)

promoter(lacI regulated)-RFP in pSEVA331-Bb


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3949
    Illegal suffix found in sequence at 1078
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3949
    Illegal SpeI site found at 1079
    Illegal PstI site found at 1093
    Illegal NotI site found at 1086
    Illegal NotI site found at 3955
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3949
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3949
    Illegal suffix found in sequence at 1079
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3949
    Illegal XbaI site found at 3964
    Illegal SpeI site found at 1079
    Illegal PstI site found at 1093
    Illegal NgoMIV site found at 2268
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K1321330 was created by restricting BBa_K1321300 and BBa_R0010 with PstI and XbaI, gel purifying the resulting fragments, ligating using T4 DNA ligase and transforming the ligation reaction into E.coli DH10B.

Source

References