Composite

Part:BBa_K1321330:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)

TetR repressed RFP in pSEVA331-Bb


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4697
    Illegal suffix found in sequence at 1826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4697
    Illegal SpeI site found at 1827
    Illegal PstI site found at 1841
    Illegal NotI site found at 1834
    Illegal NotI site found at 4703
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4697
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4697
    Illegal suffix found in sequence at 1827
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4697
    Illegal XbaI site found at 4712
    Illegal SpeI site found at 1827
    Illegal PstI site found at 1841
    Illegal NgoMIV site found at 3016
    Illegal AgeI site found at 1529
    Illegal AgeI site found at 1641
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K1321330 was created by restricting BBa_K1321300 and BBa_K577895 with PstI and XbaI, gel purifying the resulting fragments, ligating using T4 DNA ligase and transforming the ligation reaction into E.coli DH10B.


Source

References