Plasmid

Part:BBa_K1321328:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)

J-sRNA-331Bb


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4541
    Illegal EcoRI site found at 1297
    Illegal SpeI site found at 1207
    Illegal PstI site found at 1685
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1297
    Illegal EcoRI site found at 4541
    Illegal SpeI site found at 1207
    Illegal PstI site found at 1685
    Illegal NotI site found at 1678
    Illegal NotI site found at 4547
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1297
    Illegal EcoRI site found at 4541
    Illegal XhoI site found at 1145
    Illegal XhoI site found at 1151
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4541
    Illegal EcoRI site found at 1297
    Illegal SpeI site found at 1207
    Illegal PstI site found at 1685
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4541
    Illegal EcoRI site found at 1297
    Illegal XbaI site found at 4556
    Illegal SpeI site found at 1207
    Illegal PstI site found at 1685
    Illegal NgoMIV site found at 2860
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1198
    Illegal BsaI.rc site found at 987


Design Notes

Source

References