Composite

Part:BBa_K1321327:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)

pTet02


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4629
    Illegal SpeI site found at 890
    Illegal PstI site found at 1773
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4629
    Illegal SpeI site found at 890
    Illegal PstI site found at 1773
    Illegal NotI site found at 1766
    Illegal NotI site found at 4635
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4629
    Illegal SpeI site found at 890
    Illegal PstI site found at 1773
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4629
    Illegal XbaI site found at 4644
    Illegal SpeI site found at 890
    Illegal PstI site found at 1773
    Illegal NgoMIV site found at 2948
    Illegal AgeI site found at 1469
    Illegal AgeI site found at 1581
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References