Composite

Part:BBa_K1321325:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)

pLux02


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4802
    Illegal SpeI site found at 1063
    Illegal PstI site found at 1946
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4802
    Illegal SpeI site found at 1063
    Illegal PstI site found at 1946
    Illegal NotI site found at 1939
    Illegal NotI site found at 4808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4802
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4802
    Illegal SpeI site found at 1063
    Illegal PstI site found at 1946
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4802
    Illegal XbaI site found at 4817
    Illegal SpeI site found at 1063
    Illegal PstI site found at 1946
    Illegal NgoMIV site found at 3121
    Illegal AgeI site found at 1642
    Illegal AgeI site found at 1754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


Design Notes

Source

References