Coding

Part:BBa_K1321163:Design

Designed by: Gabriella Santosa   Group: iGEM14_Imperial   (2014-10-09)


NiBP driven by T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

Our NiBP came from BBa_K1151001. Our T7 vector came from an inverse PCR of the part BBa_I746909.

References