Coding

Part:BBa_K1321014:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-15)

CBDCipA with N and C-terminal linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 148
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 148
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 148
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 148
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleotide sequence was first obtained from the relevant portion of http://www.ncbi.nlm.nih.gov/nuccore/144777 then codon optimised for E. coli. The DNA sequence for RFC25 prefix and suffix was appeneded to the sequence, with an additional 4 basepairs (gatc) at the beginning and end of the sequence to allow for space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.

Source

The designed construct was ordered from as a GeneArt® String (Invitrogen™ Life Technologies).