Coding

Part:BBa_K1321012:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-10)

fMT metallothionein in Freiburg format (RFC[25])


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence from BBa_K190019 was codon optimised using the propriety software from GeneArt®. RFC[25] prefix and suffix were appended to the sequence with an additional 4 basepairs (gatc) at the beginning and end to allow space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.

Source

The designed construct was ordered from as a GeneArt® String (Invitrogen™ Life Technologies).