Coding

Part:BBa_K1321005:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-07)

Synthetic Phytochelatin EC(20) RFC[25]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The basis for the DNA sequence was taken from Bae et al (2000) [1]. This sequence was codon optimised using the propriety software from GeneArt®. RFC[25] prefix and suffix were appended to the sequence with an additional 4 basepairs (gatc) at the beginning and end to allow space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.

Source

The designed construct was ordered from as a GeneArt® Gene (Invitrogen™ Life Technologies).

References

1. Bae W, Chen W, Mulchandani A, Mehra RK (2000) Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins. Biotechnol Bioeng. 70(5):518-24.