Coding
Part:BBa_K1321001:Design
Designed by: Gabriella Santosa Group: iGEM14_Imperial (2014-08-28)
sfGFP RFC25 format
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 721 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 19
Design Notes
We used primers with Freiburg overhangs and PCR amplified.
Source
Our template was the I746909 part submitted by Cambridge 2007.