Coding
CcdB
Part:BBa_K1318000:Design
Designed by: Laurence Van Melderen Group: iGEM14_ULB-Brussels (2014-10-02)
CcdB toxin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 216
Design Notes
CcdB expression is toxic to bacteria (verified in E.coli). Express only using an inducible promoter or alongside its antidote ccdA.
Source
E.coli fertility factor (F plasmid), ccdBA operon.
References
- Miki, T., Ae Park, J., Nagao, K., Murayama, N., & Horiuchi, T. (1992). Control of segregation of chromosomal DNA by sex factor F in Escherichia coli: Mutants of DNA gyrase subunit A suppress letD (ccdB) product growth inhibition. Journal of molecular biology, 225(1), 39-52.
- Van Melderen, L. (2010). Toxin–antitoxin systems: why so many, what for? Current opinion in microbiology, 13(6), 781-785.