Coding

Part:BBa_K1316018:Design

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-10-16)

RBS + csgB curli-forming gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 516
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains an RBS upstream the gene in order to ensure proper protein translation. The part was PCRed in a way that it contains the beginning of a constitutive promoter after the gene. This aims at constructing via Golden Gate Assembly the final BioBricks BBa_K1316013 and BBa_K1316014 where csgB and csgA genes are present and a constitutive promoter was placed between them.


Source

The CDS of csgB, as well as the RBS, contained in this part were PCRed from the iGEM BioBrick BBa_K540000 Lyon team 2011.

References