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Part:BBa_K1315011:Design

Designed by: Dimitrios Michailidis   Group: iGEM14_Dundee   (2014-09-27)


Pseudomonas AutoInducer-1 response unit (lasB promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 849
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 382
    Illegal AgeI site found at 579
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.


Source

All the parts have come from existing iGEM distribution kits.

References