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Coding

Part:BBa_K1315004:Design

Designed by: Dimitrios Michailidis   Group: iGEM14_Dundee   (2014-09-25)


ydeH (dgcZ)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 775
    Illegal SapI.rc site found at 800


Design Notes

The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector. YdeH however, is toxic to E. coli cells.

Source

The source of this part is Escherichia coli MC40100 genomic DNA which was provided by the Tracy Palmer lab in the Molecular Microbiology department of the University of Dundee, UK.

References