Part:BBa_K1314013:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQ0e2426ae83f56486-partinfo-00000000-QINU

Ammonium repleted condition(left) versus nitrogen depleted condition(right) for constitutive RFP expression and nifH regulated RFP expression in A. vinelandii

Under high Ammonium level, there is strong expression of RFP under constitutive promoter. Under nitrogen depleted condition, there is no growth of A.vinelandii. This proves that the stable genome integration is successful. The mutated A.vinelandii has already lost nitrogenase gene, and thus no longer be diazotrophic.

EcoRI PstI double digestion checking our biobrick, it shows a band of insert with expected size of 5000 bp. UNIQ0e2426ae83f56486-partinfo-00000003-QINU