Part:BBa_K1314000

Ammonium repressible nifH promoter(from Azotobacter vinelandii)

This part arose from the natural version of the A. vinelandii nifH promoter with illegal restriction site removed. It is part of the transcriptional regulatory system of the nitrogenase of A. vinelandii.

Usage and Biology

The expression level of the gene under this promoter was found to be highest when nitrogen is depleted in A. vinelandii.(Trinity L. et al. , 2011) With a sufficiently low nitrogen level, the expression level from nifH promoter protein expression system is expected to be higher than that in tranditional E. coli T7 protein expression system. (2)

EcoRI PstI double digest check the gel photo here shows the expected band size of 250 bp of insert: nifH promoter

Expressing nifH promoter(Bba_K1314000) with BBa_E1010 in E. coli DH5a

Bba_K1314001 is comprised of nifH promoter and Bba_E1010. It is designed for testing nifH promoter. The right tube shows the expression of RFP using ptep promoter in Dh5a and the left tube show the expression of BbaK1314001 in DH5a. The result show that nifH promoter is not working in DH5a.

Characterization of Bba_K1314001, Bba_K1314013, Bba_K1314014 in Azotobacter vinelandii

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]